| 01/05/2010 18:00 | Bristol Rovers | Norwich | 
|
(0-2) 0-3 |
| 01/05/2010 18:00 | Carlisle | Walsall |
|
(1-1) 1-1 |
| 01/05/2010 18:00 | Charlton | Leeds |
|
(0-0) 1-0 |
| 01/05/2010 18:00 | Gillingham | Southampton |
|
(1-0) 2-1 |
| 01/05/2010 18:00 | Hartlepool | Exeter |
|
(0-1) 1-1 |
| 01/05/2010 18:00 | Huddersfield | Colchester |
|
(1-0) 2-1 |
| 01/05/2010 18:00 | Leyton Orient | Wycombe |
|
(0-0) 2-0 |
| 01/05/2010 18:00 | Milton Keynes Dons | Brighton |
|
(0-0) 0-0 |
| 01/05/2010 18:00 | Southend | Stockport |
|
(0-0) 2-1 |
| 01/05/2010 18:00 | Swindon | Brentford |
|
(2-1) 3-2 |
| 01/05/2010 18:00 | Tranmere | Millwall |
|
(1-0) 2-0 |
| 01/05/2010 18:00 | Yeovil | Oldham |
|
(1-0) 3-0 |
| 08/05/2010 18:00 | Brentford | Hartlepool |
|
(0-0) 0-0 |
| 08/05/2010 18:00 | Brighton | Yeovil |
|
(1-0) 1-0 |
| 08/05/2010 18:00 | Colchester | Leyton Orient |
|
(0-0) 1-0 |
| 08/05/2010 18:00 | Exeter | Huddersfield |
|
(1-1) 2-1 |
| 08/05/2010 18:00 | Leeds | Bristol Rovers |
|
(0-0) 2-1 |
| 08/05/2010 18:00 | Millwall | Swindon |
|
(1-1) 3-2 |
| 08/05/2010 18:00 | Norwich | Carlisle |
|
(0-2) 0-2 |
| 08/05/2010 18:00 | Oldham | Charlton |
|
(0-1) 0-2 |
| 08/05/2010 18:00 | Southampton | Southend |
|
(0-0) 3-1 |
| 08/05/2010 18:00 | Stockport | Tranmere |
|
(0-1) 0-3 |
| 08/05/2010 18:00 | Walsall | Milton Keynes Dons |
|
(0-1) 2-1 |
| 08/05/2010 18:00 | Wycombe | Gillingham |
|
(1-0) 3-0 |